Infectious Diseases II

Assay

Technology

Manufacturer/

Trade Name

Organisms/Antimicrobial

Resistance Targets

Detection Time

After Positive Growth

on Blood Culture

PNA FISH

FISH, Traffic

Light

QuickFISH

S. aureus, CoNS, E. faecalis, E. faecium, E. coli,

K. pneumoniae, P. aeruginosa, C. albicans,

C. glabrata, C. parapsilosis, C. tropicalis,

C. krusei

1.5 hr – PNA FISH

20 min – QuickFISH

– Not currently FDA

approved for Candida

spp.

PCR

Staph SR

MRSA

S. aureus, CoNS/mecA (methicillin)

1–2 hr

PCR

FilmArray

Enterococcus spp., L. monocytogenes

S. aureus, S. agalactiae, S. pneumoniae,

S. pyogenes/mecA (methicillin), vanA/B

(vancomycin)

A. baumannii, H. influenzae, N. meningitidis,

P. aeruginosa, E. cloacae, E. coli, K. oxytoca,

K. pneumoniae, Proteus spp., S. marcescens/KPC

(carbapenem)

C. albicans, C. glabrata, C. krusei,

C. parapsilosis, C. tropicalis

1 hr – Direct from

blood culture media

Vitek MS

Microflex

Several pathogens, including bacteria, yeast,

mold, mycobacteria/resistance mechanisms are in

development

10–30 min

Nanoparticles

(Microarray)

Verigene

S. aureus, S. epidermidis, S. lugdunensis,

S. anginosus, S. agalactiae, S. pneumoniae,

S. pyogenes, E. faecalis, E. faecium,

Staphylococcus spp., Streptococcus spp.,

Micrococcus spp., Listeria spp./mecA (methicillin),

vanA (vancomycin), vanB (vancomycin)

E. coli, K. pneumoniae, K. oxytoca,

P. aeruginosa, S. marcescens, Acinetobacter spp.,

Citrobacter spp., Enterobacter spp., Proteus spp./

CTX-M (ESBL), IMP (carbapenemase), KPC

(carbapenemase), NDM (carbapenemase), OXA

(carbapenemase), VIM (carbapenemase)

Candida spp. in development

2 hr – Direct from

blood culture media